Cryogenic electron tomography and elemental analysis of mitochondrial granules in human retinal ganglion cells
Structure. 2025 Jul 25:S0969-2126(25)00258-8. doi: 10.1016/j.str.2025.07.010. Online ahead of print. ABSTRACT Combining three-dimensional (3D) visualization with elemental analysis of vitrified cells can provide crucial insights into subcellular structures and elemental compositions in their native environments. We present a coordinated approach using cryogenic electron energy loss spectroscopy (cryoEELS) and cryogenic electron tomography (cryoET) to characterize the […]
Construction and characteristics of an adjustable biomechanical in vitro corneal stromal model simulating keratoconus pathological features
Mater Today Bio. 2025 Jul 23;34:102130. doi: 10.1016/j.mtbio.2025.102130. eCollection 2025 Oct. ABSTRACT Keratoconus (KC) is a prevalent ectatic corneal disease influenced by corneal biomechanics, though its pathogenesis remains unclear. Constructing an in vitro corneal model with adjustable biomechanics is essential for studying keratoconus. In this study, a KC disease group (L group) was created using […]
MiRNA-122-5p Promotes Retinal Ganglion Cell Oxidative Damage by Targeting DJ-1 in Hyperglycemic Retina
Free Radic Biol Med. 2025 Aug 2:S0891-5849(25)00869-X. doi: 10.1016/j.freeradbiomed.2025.07.054. Online ahead of print. ABSTRACT Mitochondrial dysfunction, induced by prolonged hyperglycemia, is widely regarded as a central factor in oxidative stress and retinal ganglion cell (RGC) degeneration in diabetic retinopathy (DR). DJ-1 (PARK7) acts as a crucial antioxidant defense mechanism, stabilizing mitochondrial structure and redox balance. […]
Adipose-derived mesenchymal stem cells and retinal pigment epithelial cells interactions in a stress environment via tunneling nanotubes
PLoS One. 2025 Aug 4;20(8):e0329672. doi: 10.1371/journal.pone.0329672. eCollection 2025. ABSTRACT This study aims to demonstrate the formation of tunneling tubes (TNTs) between adipose-derived mesenchymal stem cells (AdMSCs) and retinal pigment epithelial cells (RPE-1) and their alterations in response to experimental stress conditions. Serum starvation was employed as a stress condition to induce TNTs between the […]
Digitalization of surgical features improves surgical accuracy via surgeon guidance and robotization
NPJ Digit Med. 2025 Aug 2;8(1):497. doi: 10.1038/s41746-025-01887-6. ABSTRACT The evolution of surgical techniques aims to augment surgeons’ capabilities through digital guidance and robotization for higher precision and consistency. Currently, surgeries heavily rely on surgeon’s experience and visual judgment, causing operation variations. Artificial intelligence (AI) offers a solution by extracting and digitizing surgical trajectories and […]
IVF add-ons: effectiveness, efficiency, safety, and additional costs – A position statement by the SIFES-MR
Eur J Obstet Gynecol Reprod Biol. 2025 Jul 29;313:114609. doi: 10.1016/j.ejogrb.2025.114609. Online ahead of print. ABSTRACT OBJECTIVE: To critically appraise IVF add-ons, based on current evidence, international recommendations, and expert consensus from SIFES-MR. STUDY DESIGN: Consensus developed by a multidisciplinary panel of Italian reproductive medicine experts over two years, based on clinical expertise, revision of […]
Boronic acid derivatives inhibit Candida albicans growth by compromising energy metabolism
J Adv Res. 2025 Jul 28:S2090-1232(25)00573-9. doi: 10.1016/j.jare.2025.07.044. Online ahead of print. ABSTRACT INTRODUCTION: Fungal infections represent a global health concern with significant implications for human well-being. Among them, refractory and sight-threatening fungal keratitis (FK) poses a serious threat to ocular health. This underscores an urgent need for developing novel antifungal agents to meet clinical […]
Mitochondrial Disorder: Kearns-Sayre Syndrome
Adv Exp Med Biol. 2025;1467:173-175. doi: 10.1007/978-3-031-72230-1_30. ABSTRACT Mitochondrial DNA (mtDNA) deletions can be identified, and rarely point mutation. PMID:40736832 | DOI:10.1007/978-3-031-72230-1_30
Drug-Induced Retinal Toxicity
Adv Exp Med Biol. 2025;1467:253-262. doi: 10.1007/978-3-031-72230-1_50. ABSTRACT Despite the protective blood-ocular barrier, various drugs can induce retinal damage, manifesting as retinal pigment epithelium dysfunction, photoreceptor degeneration, vascular abnormalities, ganglion cell damage, optic nerve impairment, or uveitis. These toxic effects are often linked to mechanisms such as oxidative stress, mitochondrial dysfunction, and cytoskeletal disruption. Early […]
Mitochondrial Disorder: Maternally Inherited Diabetes and Deafness
Adv Exp Med Biol. 2025;1467:177-180. doi: 10.1007/978-3-031-72230-1_31. ABSTRACT Patients with maternally inherited diabetes and deafness (MIDD) have insulin-dependent diabetes with relatively low BMI; usually the onset of the diabetes is during the third or fourth decade of life and it is associated with progressive neurosensory deafness. PMID:40736833 | DOI:10.1007/978-3-031-72230-1_31