Mitophagy and mitochondrion-related expression profiles in response to physiological and pathological hypoxia in the corneal epithelium
Genomics. 2023 Oct 31:110739. doi: 10.1016/j.ygeno.2023.110739. Online ahead of print. ABSTRACT To study the mitochondrial and cellular responses to physiological and pathological hypoxia, corneal epithelial cells were preconditioned under 21% O2, 8% O2 or 1% O2. The cell survival rate, mitochondrial fluorescence and mitophagy flux were quantified using flow cytometry. After RNA sequencing, gene set […]
Single-cell characterization of macrophages in uveal melanoma uncovers transcriptionally heterogeneous subsets conferring poor prognosis and aggressive behavior
Exp Mol Med. 2023 Nov 1. doi: 10.1038/s12276-023-01115-9. Online ahead of print. ABSTRACT Uveal melanoma (UM) is the most frequent primary intraocular malignancy with high metastatic potential and poor prognosis. Macrophages represent one of the most abundant infiltrating immune cells with diverse functions in cancers. However, the cellular heterogeneity and functional diversity of macrophages in […]
Neutral sphingomyelinase inhibition promotes local and network degeneration in vitro and in vivo
Cell Commun Signal. 2023 Oct 30;21(1):305. doi: 10.1186/s12964-023-01291-1. ABSTRACT BACKGROUND: Cell-to-cell communication is vital for tissues to respond, adapt, and thrive in the prevailing milieu. Several mechanisms mediate intercellular signaling, including tunneling nanotubes, gap junctions, and extracellular vesicles (EV). Depending on local and systemic conditions, EVs may contain cargoes that promote survival, neuroprotection, or pathology. […]
Episodic live imaging of cone photoreceptor maturation in GNAT2-EGFP retinal organoids
Dis Model Mech. 2023 Oct 30:dmm.050193. doi: 10.1242/dmm.050193. Online ahead of print. ABSTRACT Fluorescent reporter pluripotent stem cell (PSC) derived retinal organoids are powerful tools to investigate cell type-specific development and disease phenotypes. When combined with live imaging, they enable direct and repeated observation of cell behaviors within a developing retinal tissue. Here, we generated […]
Severe Lactic Acidosis Caused by Thiamine Deficiency in a Child with Relapsing Acute Lymphoblastic Leukemia: A Case Report
Children (Basel). 2023 Sep 26;10(10):1602. doi: 10.3390/children10101602. ABSTRACT Lactic acidosis is characterized by an excessive production of lactic acid or by its impaired clearance. Thiamine deficiency is an uncommon cause of lactic acidosis, especially in countries where malnutrition is rare. We describe the case of a 5-year-old boy who presented with a central nervous system […]
Mitophagy in Astrocytes Is Required for the Health of Optic Nerve
Cells. 2023 Oct 20;12(20):2496. doi: 10.3390/cells12202496. ABSTRACT Mitochondrial dysfunction in astrocytes has been implicated in the development of various neurological disorders. Mitophagy, mitochondrial autophagy, is required for proper mitochondrial function by preventing the accumulation of damaged mitochondria. The importance of mitophagy, specifically in the astrocytes of the optic nerve (ON), has been little studied. We […]
The diagnostic accuracy of photopic negative responses evoked by broadband and chromatic stimuli in a clinically heterogeneous population
Doc Ophthalmol. 2023 Oct 27. doi: 10.1007/s10633-023-09956-5. Online ahead of print. ABSTRACT PURPOSE: To compare the diagnostic accuracy of the photopic negative response (PhNR) elicited by red-blue (RB) and white-white (WW) stimuli, for detection of retinal ganglion cell (RGC) dysfunction in a heterogeneous clinical cohort. METHODS: Adults referred for electrophysiological investigations were recruited consecutively for […]
Cigarette smoke causes a bioenergetic crisis in RPE cells involving the downregulation of HIF-1α under normoxia
Cell Death Discov. 2023 Oct 25;9(1):398. doi: 10.1038/s41420-023-01695-5. ABSTRACT Age-related macular degeneration (AMD) is the most common blinding disease in the elderly population. However, there are still many uncertainties regarding the pathophysiology at the molecular level. Currently, impaired energy metabolism in retinal pigment epithelium (RPE) cells is discussed as one major hallmark of early AMD […]
Targeting Ferroptosis in Bone-Related Diseases: Facts and Perspectives
J Inflamm Res. 2023 Oct 18;16:4661-4677. doi: 10.2147/JIR.S432111. eCollection 2023. ABSTRACT Ferroptosis is a new cell fate decision discovered in recent years. Unlike apoptosis, autophagy or pyroptosis, ferroptosis is characterized by iron-dependent lipid peroxidation and mitochondrial morphological changes. Ferroptosis is involved in a variety of physiological and pathological processes. Since its discovery, ferroptosis has been […]
Blocking Mitochondrial Pyruvate Transport Alters Corneal Myofibroblast Phenotype: A New Target for Treating Fibrosis
Invest Ophthalmol Vis Sci. 2023 Oct 3;64(13):36. doi: 10.1167/iovs.64.13.36. ABSTRACT PURPOSE: The purpose of this study was to critically test the hypothesis that mitochondrial pyruvate carrier (MPC) function is essential for maintenance of the corneal myofibroblast phenotype in vitro and in vivo. METHODS: Protein and mRNA for canonical profibrotic markers were assessed in cultured cat […]