A comprehensive analysis of library preparation methods shows high heterogeneity of extrachromosomal circular DNA but distinct chromosomal amount levels reflecting different cell states
Analyst. 2023 Nov 21. doi: 10.1039/d3an01300f. Online ahead of print. ABSTRACT Extrachromosomal circular DNA (eccDNA) was discovered several decades ago, but little is known about its function. With the development of sequencing technology, several library preparation methods have been developed to elucidate the biogenesis and function of eccDNA. However, different treatment methods have certain biases […]
Targeting the Mitochondrial Chaperone TRAP1 Alleviates Vascular Pathologies in Ischemic Retinopathy
Adv Sci (Weinh). 2023 Nov 20:e2302776. doi: 10.1002/advs.202302776. Online ahead of print. ABSTRACT Activation of hypoxia-inducible factor 1α (HIF1α) contributes to blood-retinal barrier (BRB) breakdown and pathological neovascularization responsible for vision loss in ischemic retinal diseases. During disease progression, mitochondrial biology is altered to adapt to the ischemic environment created by initial vascular dysfunction, but […]
Progress in diagnosis and treatment of Leber’s hereditary optic neuropathy
J Mol Med (Berl). 2023 Nov 20. doi: 10.1007/s00109-023-02389-2. Online ahead of print. ABSTRACT Leber’s hereditary optic neuropathy (LHON) is a mitochondrial genetic disease with central vision loss as the main symptom. It is one of the diseases that cause vision loss and optic atrophy in young and middle-aged people. The mutations of these three […]
Iron derived from NCOA4-mediated ferritinophagy causes cellular senescence via the cGAS-STING pathway
Cell Death Discov. 2023 Nov 18;9(1):419. doi: 10.1038/s41420-023-01712-7. ABSTRACT Cellular senescence is a hallmark of aging and has been linked to age-related diseases. Age-related macular degeneration (AMD), the most common aging-related retinal disease, is prospectively associated with retinal pigment epithelial (RPE) senescence. However, the mechanism of RPE cell senescence remains unknown. In this study, tert-butyl […]
Retinal ischemia-reperfusion injury induces intense lipid synthesis and remodeling
Biochem Biophys Res Commun. 2023 Nov 10;689:149232. doi: 10.1016/j.bbrc.2023.149232. Online ahead of print. ABSTRACT The retina is a high-metabolism tissue composed of various cell types with complex functions that relies heavily on the blood supply to maintain homeostasis. Retinal ischemia-reperfusion injury is a critical pathogenic mechanism in glaucoma, and changes in lipid molecules may lead […]
Deferoxamine attenuates visual impairment in retinal ischemia‒reperfusion via inhibiting ferroptosis
Sci Rep. 2023 Nov 17;13(1):20145. doi: 10.1038/s41598-023-46104-0. ABSTRACT Retinal ischemia‒reperfusion (I/R) injury can cause significant damage to human retinal neurons, greatly compromising their functions. Existing interventions have been proven to have little effect. Ferroptosis is a newly discovered type of programmed cell death that has been found to be involved in the process of ischemia‒reperfusion […]
Mitochondria-targeted SkQ1 nanoparticles for dry eye disease: Inhibiting NLRP3 inflammasome activation by preventing mitochondrial DNA oxidation
J Control Release. 2023 Nov 14:S0168-3659(23)00738-1. doi: 10.1016/j.jconrel.2023.11.021. Online ahead of print. ABSTRACT Dry eye disease (DED) is a multifactorial ocular surface disorder mutually promoted by reactive oxygen species (ROS) and ocular surface inflammation. NLRP3 is the key regulator for inducing ocular surface inflammation in DED. However, the mechanism by which ROS influences the bio-effects […]
Disruption of CFAP418 interaction with lipids causes widespread abnormal membrane-associated cellular processes in retinal degenerations
JCI Insight. 2023 Nov 16:e162621. doi: 10.1172/jci.insight.162621. Online ahead of print. ABSTRACT Syndromic ciliopathies and retinal degenerations are large heterogeneous groups of genetic diseases. Pathogenic variants in the CFAP418 gene may cause both disorders, and its protein sequence is evolutionarily conserved. However, the disease mechanism underlying CFAP418 mutations has not been explored. Here, we apply […]
LKB1-SIK2 loss drives uveal melanoma proliferation and hypersensitivity to SLC8A1 and ROS inhibition
EMBO Mol Med. 2023 Nov 15:e17719. doi: 10.15252/emmm.202317719. Online ahead of print. ABSTRACT Metastatic uveal melanomas are highly resistant to all existing treatments. To address this critical issue, we performed a kinome-wide CRISPR-Cas9 knockout screen, which revealed the LKB1-SIK2 module in restraining uveal melanoma tumorigenesis. Functionally, LKB1 loss enhances proliferation and survival through SIK2 inhibition […]
Expression of YAP suppresses cell proliferation and elevates the sensitivity of chemotherapy in retinoblastoma cells through lipid-peroxidation induced ferroptosis
Chin Clin Oncol. 2023 Oct;12(5):52. doi: 10.21037/cco-23-97. ABSTRACT BACKGROUND: Retinoblastoma (RB) is a retinal cancer most commonly occurred in young children. Cisplatin and etoposide had been confirmed as chemotherapy drugs in the treatment of RB, even though the phenomenon of chemotherapeutic resistance has been occurring in clinical treatment frequently. RB has been reported to be […]