Electroacupuncture Slows Experimental Myopia Progression by Improving Retinal Mitochondrial Function: A Study Based on Single-Cell RNA Sequencing
Adv Biol (Weinh). 2024 Oct 15:e2400269. doi: 10.1002/adbi.202400269. Online ahead of print. ABSTRACT This study aimed to establish a complete atlas of retinal cells in lens-induced myopia (LIM) and electroacupuncture (EA) intervention by single-cell RNA sequencing (scRNA-seq) and to explore the potential mechanism of EA in improving experimental myopia progression in guinea pigs. scRNA-seq is […]
Hyperglycemia-depleted glutamine contributes to the pathogenesis of diabetic corneal endothelial dysfunction
Exp Eye Res. 2024 Oct 11:110124. doi: 10.1016/j.exer.2024.110124. Online ahead of print. ABSTRACT Diabetic mellitus causes various complications, including the corneal endothelial dysfunction (CED) that leads to corneal edema and vision loss, especially in the DM patients with intraocular surgeries. However, the pathogenic mechanism of hyperglycemia-caused CED remains incomplete understood. Here we firstly screened and […]
Nrf2 mitigates sepsis-associated encephalopathy-induced hippocampus ferroptosis via modulating mitochondrial dynamic homeostasis
Int Immunopharmacol. 2024 Oct 12;143(Pt 1):113331. doi: 10.1016/j.intimp.2024.113331. Online ahead of print. ABSTRACT Sepsis-associated encephalopathy (SAE) is a serious neurological complication accompanied with acute and long-term cognitive dysfunction. Ferroptosis is a newly discovered type of cell death that is produced by iron-dependent lipid peroxidation. Emerging evidence suggests that ferroptosis is involved in SAE. The nuclear […]
COL8A2 Activation Enhances Function of Corneal Endothelial Cells through HIPPO Signaling/Mitochondria Pathway
Matrix Biol. 2024 Oct 10:S0945-053X(24)00122-7. doi: 10.1016/j.matbio.2024.10.001. Online ahead of print. ABSTRACT Corneal endothelial cells (CECs) are essential for maintaining corneal transparency and hydration through their barrier and pump functions. The COL8A2 gene encodes a component of the extracellular matrix of the cornea, which is crucial for the normal functioning of these cells. Mutations in […]
Retinal Organoid Microenvironment Enhanced Bioactivities of Microglia-Like Cells Derived From HiPSCs
Invest Ophthalmol Vis Sci. 2024 Oct 1;65(12):19. doi: 10.1167/iovs.65.12.19. ABSTRACT PURPOSE: Microglia-like cells derived from stem cells (iMG) provide a plentiful cell source for studying the functions of microglia in both normal and pathological conditions. Our goal is to establish a simplified and effective method for generating iMG in a precisely defined system. Additionally, we […]
TGF-β effects on adipogenesis of 3T3-L1 cells differ in 2D and 3D cell culture conditions
FEBS Open Bio. 2024 Oct 8. doi: 10.1002/2211-5463.13890. Online ahead of print. ABSTRACT The TGF-β superfamily plays a pivotal role in the regulation of adipogenesis, but little is known about the potential differential role of the three isoforms of TGF-β, TGF-β-1~3. To further elucidate their role, two-dimensionally (2D) and three-dimensionally (3D) cultured 3T3-L1 mouse preadipocytes […]
The influence of polyacrylamide gel substrate elasticity on primary cultures of rat retinal ganglion cells
Exp Eye Res. 2024 Oct 5:110116. doi: 10.1016/j.exer.2024.110116. Online ahead of print. ABSTRACT In vitro primary cell culture models of retinal ganglion cells (RGC) are widely used to study pathomechanisms of diseases such as glaucoma. The biomechanic interaction with the culture substrate is known to influence core cellular functions. RGC cultures, however, are usually grown […]
Lymphatic-specific methyltransferase-like 3-mediated m6A modification drives vascular patterning through prostaglandin metabolism reprogramming
MedComm (2020). 2024 Oct 4;5(10):e728. doi: 10.1002/mco2.728. eCollection 2024 Oct. ABSTRACT Lymphangiogenesis plays a pivotal role in the pathogenesis of various vascular disorders, including ocular vascular diseases and cancers. Deregulation of N 6-methyladenosine (m6A) modification has been identified as a key contributor to human diseases. However, the specific involvement of m6A modification in lymphatic remodeling […]
Recognizing Leber’s Hereditary Optic Neuropathy to avoid delayed diagnosis and misdiagnosis
Front Neurol. 2024 Sep 19;15:1466275. doi: 10.3389/fneur.2024.1466275. eCollection 2024. ABSTRACT Leber’s Hereditary Optic Neuropathy (LHON) is a maternally inherited optic nerve disease primarily caused by mutations in mitochondrial DNA (mtDNA). The peak of onset is typically between 15 and 30 years, but variability exists. Misdiagnosis, often as inflammatory optic neuritis, delays treatment, compounded by challenges […]
Recent Advances in NLRP3 Inflammasome in Corneal Diseases: Preclinical Insights and Therapeutic Implications
Ocul Surf. 2024 Sep 30:S1542-0124(24)00103-4. doi: 10.1016/j.jtos.2024.09.007. Online ahead of print. ABSTRACT NLRP3 inflammasome is a cytosolic multiprotein complex formed in response to exogenous environmental stress and cellular damage. The three major components of the NLRP3 inflammasome are the innate immunoreceptor protein NLRP3, the adapter protein apoptosis-associated speck-like protein containing a C-terminal caspase activation and […]