Evaluation of transcriptomic changes after photobiomodulation in spinal cord injury
Sci Rep. 2025 Jan 25;15(1):3193. doi: 10.1038/s41598-025-87300-4. ABSTRACT Spinal cord injury (SCI) is a significant cause of lifelong disability, with no available disease-modifying treatments to promote neuroprotection and axon regeneration after injury. Photobiomodulation (PBM) is a promising therapy which has proven effective at restoring lost function after SCI in pre-clinical models. However, the precise mechanism […]
Metabolic Reprogramming of Neutrophils in the Tumor Microenvironment: Emerging Therapeutic Targets
Cancer Lett. 2025 Jan 23:217466. doi: 10.1016/j.canlet.2025.217466. Online ahead of print. ABSTRACT Neutrophils are pivotal in the immune system and have been recognized as significant contributors to cancer development and progression. These cells undergo metabolic reprogramming in response to various stimulus, including infections, diseases, and the tumor microenvironment (TME). Under normal conditions, neutrophils primarily rely […]
Protocol for real-time assessment of energy metabolism in dissociated mouse retinal photoreceptors using a SeahorseXFe24 analyzer
STAR Protoc. 2025 Jan 23;6(1):103595. doi: 10.1016/j.xpro.2025.103595. Online ahead of print. ABSTRACT Defects in retinal metabolism have been linked to the onset and progression of various retinal diseases. Herein, we provide a protocol for measuring bioenergetics in dissociated mouse retinal photoreceptors. We outline detailed instructions for obtaining morphologically intact and viable photoreceptor cells from adult […]
Mitochondrial transplantation: a promising strategy for the treatment of retinal degenerative diseases
Neural Regen Res. 2024 Dec 7. doi: 10.4103/NRR.NRR-D-24-00851. Online ahead of print. ABSTRACT The retina, a crucial neural tissue, is responsible for transforming light signals into visual information, a process that necessitates a significant amount of energy. Mitochondria, the primary powerhouses of the cell, play an integral role in retinal physiology by fulfilling the high-energy […]
Derivation and Characterization of Isogenic OPA1 Mutant and Control Human Pluripotent Stem Cell Lines
Cells. 2025 Jan 17;14(2):137. doi: 10.3390/cells14020137. ABSTRACT Dominant optic atrophy (DOA) is the most commonly inherited optic neuropathy. The majority of DOA is caused by mutations in the OPA1 gene, which encodes a dynamin-related GTPase located to the mitochondrion. OPA1 has been shown to regulate mitochondrial dynamics and promote fusion. Within the mitochondrion, proteolytically processed […]
Chronic degenerative failure of salivary glands can be reversed through restoring mitochondrial function
bioRxiv [Preprint]. 2025 Jan 2:2024.12.31.630834. doi: 10.1101/2024.12.31.630834. ABSTRACT There are no therapies for reversing chronic organ degeneration. Non-healing degenerative wounds are thought to be irreparable, in part, by the inability of the tissue to respond to reparative stimuli. As such, treatments are typically aimed at slowing tissue degeneration or replacing cells through transplantation. Building on […]
ROCK Inhibitor Enhances Resilience Against Metabolic Stress Through Increasing Bioenergetic Capacity in Corneal Endothelial Cells
Invest Ophthalmol Vis Sci. 2025 Jan 2;66(1):51. doi: 10.1167/iovs.66.1.51. ABSTRACT PURPOSE: To investigate the effect of Rho-associated protein kinase (ROCK) inhibitor Y27632 on bioenergetic capacity and resilience of corneal endothelial cells (CECs) under metabolic stress. METHODS: Bovine CECs (BCECs) were treated with Y27632 and subjected to bioenergetic profiling using the Seahorse XFp Analyzer. The effects […]
Mitochondrial Localization of Antioxidant Nanodrug Suppresses Ocular Inflammation by Alleviating Oxidative Stress on Cells
Chemistry. 2025 Jan 20:e202402441. doi: 10.1002/chem.202402441. Online ahead of print. ABSTRACT External environments (e.g., pollutants, irritants, ultraviolet radiation, etc) probably activate oxidative stress on the ocular surface, further leading to inflammatory responses and cellular apoptosis. For treating ophthalmic diseases, one of the strategies is to regulate oxidative stress through antioxidants. Here we conjugate a polyphenolic […]
Antioxidant MitoQ increases viability of human corneal endothelial cells with congenital hereditary endothelial dystrophy-associated SLC4A11 mutations
Ophthalmic Genet. 2025 Jan 20:1-8. doi: 10.1080/13816810.2025.2450455. Online ahead of print. ABSTRACT PURPOSE: To assess the impact of MitoQ, a mitochondria-targeted antioxidant, on viability of human corneal endothelial cell (hCEnC) lines expressing SLC4A11 mutations associated with congenital hereditary endothelial dystrophy (CHED) and Fuchs endothelial corneal dystrophy type 4 (FECD4). METHODS: SLC4A11 wildtype (SLC4A11WT) and mutant […]
Family and genetic counseling in Leber hereditary optic neuropathy
Ophthalmic Genet. 2025 Jan 20:1-9. doi: 10.1080/13816810.2025.2451175. Online ahead of print. ABSTRACT AIM: Leber hereditary optic neuropathy (LHON) predominantly manifests during adolescence or young adulthood, resulting in sudden and profound vision loss in individuals who previously had normal vision. This abrupt change significantly impacts daily life, necessitating emotional support, counseling and low-vision rehabilitative services to […]