Retinal Organoid Microenvironment Enhanced Bioactivities of Microglia-Like Cells Derived From HiPSCs
Invest Ophthalmol Vis Sci. 2024 Oct 1;65(12):19. doi: 10.1167/iovs.65.12.19. ABSTRACT PURPOSE: Microglia-like cells derived from stem cells (iMG) provide a plentiful cell source for studying the functions of microglia in both normal and pathological conditions. Our goal is to establish a simplified and effective method for generating iMG in a precisely defined system. Additionally, we […]
TGF-β effects on adipogenesis of 3T3-L1 cells differ in 2D and 3D cell culture conditions
FEBS Open Bio. 2024 Oct 8. doi: 10.1002/2211-5463.13890. Online ahead of print. ABSTRACT The TGF-β superfamily plays a pivotal role in the regulation of adipogenesis, but little is known about the potential differential role of the three isoforms of TGF-β, TGF-β-1~3. To further elucidate their role, two-dimensionally (2D) and three-dimensionally (3D) cultured 3T3-L1 mouse preadipocytes […]
The influence of polyacrylamide gel substrate elasticity on primary cultures of rat retinal ganglion cells
Exp Eye Res. 2024 Oct 5:110116. doi: 10.1016/j.exer.2024.110116. Online ahead of print. ABSTRACT In vitro primary cell culture models of retinal ganglion cells (RGC) are widely used to study pathomechanisms of diseases such as glaucoma. The biomechanic interaction with the culture substrate is known to influence core cellular functions. RGC cultures, however, are usually grown […]
Lymphatic-specific methyltransferase-like 3-mediated m6A modification drives vascular patterning through prostaglandin metabolism reprogramming
MedComm (2020). 2024 Oct 4;5(10):e728. doi: 10.1002/mco2.728. eCollection 2024 Oct. ABSTRACT Lymphangiogenesis plays a pivotal role in the pathogenesis of various vascular disorders, including ocular vascular diseases and cancers. Deregulation of N 6-methyladenosine (m6A) modification has been identified as a key contributor to human diseases. However, the specific involvement of m6A modification in lymphatic remodeling […]
Recognizing Leber’s Hereditary Optic Neuropathy to avoid delayed diagnosis and misdiagnosis
Front Neurol. 2024 Sep 19;15:1466275. doi: 10.3389/fneur.2024.1466275. eCollection 2024. ABSTRACT Leber’s Hereditary Optic Neuropathy (LHON) is a maternally inherited optic nerve disease primarily caused by mutations in mitochondrial DNA (mtDNA). The peak of onset is typically between 15 and 30 years, but variability exists. Misdiagnosis, often as inflammatory optic neuritis, delays treatment, compounded by challenges […]
Recent Advances in NLRP3 Inflammasome in Corneal Diseases: Preclinical Insights and Therapeutic Implications
Ocul Surf. 2024 Sep 30:S1542-0124(24)00103-4. doi: 10.1016/j.jtos.2024.09.007. Online ahead of print. ABSTRACT NLRP3 inflammasome is a cytosolic multiprotein complex formed in response to exogenous environmental stress and cellular damage. The three major components of the NLRP3 inflammasome are the innate immunoreceptor protein NLRP3, the adapter protein apoptosis-associated speck-like protein containing a C-terminal caspase activation and […]
Mitochondrial ribosomal proteins in metastasis and their potential use as prognostic and therapeutic targets
Cancer Metastasis Rev. 2024 Oct 1. doi: 10.1007/s10555-024-10216-4. Online ahead of print. ABSTRACT The mitochondrion is an essential cell organelle known as the powerhouse of the cell. Mitochondrial ribosomal proteins (MRPs) are nuclear encoded, synthesised in the cytoplasm but perform their main functions in the mitochondria, which includes translation, transcription, cell death and maintenance. However, […]
Exposure to aircraft noise exacerbates cardiovascular and oxidative damage in three mouse models of diabetes
Eur J Prev Cardiol. 2024 Oct 1:zwae320. doi: 10.1093/eurjpc/zwae320. Online ahead of print. ABSTRACT BACKGROUND: Epidemiology links noise to increased risk of metabolic diseases like diabetes and obesity. Translational studies in humans and experimental animals showed that noise causes reactive oxygen species (ROS)-mediated cardiovascular damage. The interaction between noise and diabetes, specifically potential additive adverse […]
ROCK inhibitor enhances mitochondrial transfer via tunneling nanotubes in retinal pigment epithelium
Theranostics. 2024 Sep 9;14(15):5762-5777. doi: 10.7150/thno.96508. eCollection 2024. ABSTRACT Rationale: Tunnel nanotube (TNT)-mediated mitochondrial transport is crucial for the development and maintenance of multicellular organisms. Despite numerous studies highlighting the significance of this process in both physiological and pathological contexts, knowledge of the underlying mechanisms is still limited. This research focused on the role of […]
Adaptive protein synthesis in genetic models of copper deficiency and childhood neurodegeneration
bioRxiv [Preprint]. 2024 Sep 12:2024.09.09.612106. doi: 10.1101/2024.09.09.612106. ABSTRACT Rare inherited diseases caused by mutations in the copper transporters SLC31A1 (CTR1) or ATP7A induce copper deficiency in the brain and throughout the body, causing seizures and neurodegeneration in infancy. The mechanistic underpinnings of such neuropathology remains unclear. Here, we characterized the molecular mechanisms by which neuronal […]