Effect of PPARγ on oxidative stress in diabetes-related dry eye
Exp Eye Res. 2023 May 9:109498. doi: 10.1016/j.exer.2023.109498. Online ahead of print. ABSTRACT Oxidative stress is closely associated with diabetes and can cause free radical accumulation and eventually lead to ocular surface tissue damage. The purpose of this study was to investigate peroxisome proliferator-activated receptor-γ (PPARγ) expression in the lacrimal gland (LG), meibomian gland, and […]
Dysregulation of DNA repair genes in Fuchs endothelial corneal dystrophy
Exp Eye Res. 2023 May 9:109499. doi: 10.1016/j.exer.2023.109499. Online ahead of print. ABSTRACT Fuchs Endothelial Corneal Dystrophy (FECD), a late-onset oxidative stress disorder, is the most common cause of corneal endothelial degeneration and is genetically associated with CTG repeat expansion in TCF4. We previously reported accumulation of nuclear (nDNA) and mitochondrial (mtDNA) damage in FECD. […]
Hydrogel assisted photoreceptor delivery inhibits material transfer
Biomaterials. 2023 Apr 29;298:122140. doi: 10.1016/j.biomaterials.2023.122140. Online ahead of print. ABSTRACT Cell therapy holds tremendous promise for vision restoration; yet donor cell survival and integration continue to limit efficacy of these strategies. Transplanted photoreceptors, which mediate light sensitivity in the retina, transfer cytoplasmic components to host photoreceptors instead of integrating into the tissue. Donor cell […]
Spatiotemporal Coordination of RPE Cell Quality by Extracellular Vesicle miR-494-3p Via Competitive Interplays With SIRT3 or PTEN
Invest Ophthalmol Vis Sci. 2023 May 1;64(5):9. doi: 10.1167/iovs.64.5.9. ABSTRACT PURPOSE: To reveal the molecular mechanism underlying degeneration in human retinal pigment epithelial (hRPE) cells with dysfunctional mitochondrial homeostasis. METHODS: The expression of recently identified miR-494-3p in extracellular vesicles (EV) released from induced-pluripotential-stem-cell-derived human RPE (iPS-hRPE), during coculture with macrophages (Mps) was investigated in iPS-hRPE […]
Perspectives of Rare Disease Experts on Newborn Genome Sequencing
JAMA Netw Open. 2023 May 1;6(5):e2312231. doi: 10.1001/jamanetworkopen.2023.12231. ABSTRACT IMPORTANCE: Newborn genome sequencing (NBSeq) can detect infants at risk for treatable disorders currently undetected by conventional newborn screening. Despite broad stakeholder support for NBSeq, the perspectives of rare disease experts regarding which diseases should be screened have not been ascertained. OBJECTIVE: To query rare disease […]
Vitamin C protects retinal ganglion cells via SPP1 in glaucoma and after optic nerve damage
Life Sci Alliance. 2023 May 9;6(8):e202301976. doi: 10.26508/lsa.202301976. Print 2023 Aug. ABSTRACT Glaucoma is a common neurodegenerative disorder characterized by retinal ganglion cell death, astrocyte reactivity in the optic nerve, and vision loss. Currently, lowering the intraocular pressure (IOP) is the first-line treatment, but adjuvant neuroprotective approaches would be welcome. Vitamin C possesses neuroprotective activities […]
Artesunate protects against ocular fibrosis by suppressing fibroblast activation and inducing mitochondria-dependent ferroptosis
FASEB J. 2023 Jun;37(6):e22954. doi: 10.1096/fj.202201867R. ABSTRACT Artesunate, a derivative from extracts of Artemisia annua, has recently been reported to alleviate fibrosis recently. Here, in this study, we sought to determine the anti-fibrosis effect of artesunate in rabbit glaucoma filtration surgery (GFS) model and illuminate underlying mechanisms. Our results showed that artesunate subconjunctival injection alleviated […]
GSK-3β-dependent Nrf2 antioxidant response modulates ferroptosis of lens epithelial cells in age-related cataract
Free Radic Biol Med. 2023 May 6:S0891-5849(23)00399-4. doi: 10.1016/j.freeradbiomed.2023.04.022. Online ahead of print. ABSTRACT Oxidative stress-induced lens epithelial cells (LECs) death plays a pivotal role in age-related cataract (ARC) with severe visual impairment, in which ferroptosis is gradually receiving numerous attention resulting from lipid peroxide accumulation and reactive oxygen species (ROS) overproduction. However, the essential […]
The roles of autophagy and mitophagy in corneal pathology: current knowledge and future perspectives
Front Med (Lausanne). 2023 Apr 21;10:1064938. doi: 10.3389/fmed.2023.1064938. eCollection 2023. ABSTRACT The cornea is the clear dome that covers the front portion of the globe. The primary functions of the cornea are to promote the refraction of light and to protect the eye from invading pathogens, both of which are essential for the preservation of […]
Dual leucine zipper kinase is necessary for retinal ganglion cell axonal regeneration in Xenopus laevis
PNAS Nexus. 2023 Mar 30;2(5):pgad109. doi: 10.1093/pnasnexus/pgad109. eCollection 2023 May. ABSTRACT Retinal ganglion cell (RGC) axons of the African clawed frog, Xenopus laevis, unlike those of mammals, are capable of regeneration and functional reinnervation of central brain targets following injury. Here, we describe a tadpole optic nerve crush (ONC) procedure and assessments of brain reinnervation […]