Cigarette smoke causes a bioenergetic crisis in RPE cells involving the downregulation of HIF-1α under normoxia
Cell Death Discov. 2023 Oct 25;9(1):398. doi: 10.1038/s41420-023-01695-5. ABSTRACT Age-related macular degeneration (AMD) is the most common blinding disease in the elderly population. However, there are still many uncertainties regarding the pathophysiology at the molecular level. Currently, impaired energy metabolism in retinal pigment epithelium (RPE) cells is discussed as one major hallmark of early AMD […]
Targeting Ferroptosis in Bone-Related Diseases: Facts and Perspectives
J Inflamm Res. 2023 Oct 18;16:4661-4677. doi: 10.2147/JIR.S432111. eCollection 2023. ABSTRACT Ferroptosis is a new cell fate decision discovered in recent years. Unlike apoptosis, autophagy or pyroptosis, ferroptosis is characterized by iron-dependent lipid peroxidation and mitochondrial morphological changes. Ferroptosis is involved in a variety of physiological and pathological processes. Since its discovery, ferroptosis has been […]
Blocking Mitochondrial Pyruvate Transport Alters Corneal Myofibroblast Phenotype: A New Target for Treating Fibrosis
Invest Ophthalmol Vis Sci. 2023 Oct 3;64(13):36. doi: 10.1167/iovs.64.13.36. ABSTRACT PURPOSE: The purpose of this study was to critically test the hypothesis that mitochondrial pyruvate carrier (MPC) function is essential for maintenance of the corneal myofibroblast phenotype in vitro and in vivo. METHODS: Protein and mRNA for canonical profibrotic markers were assessed in cultured cat […]
PM10 and Pseudomonas aeruginosa: effects on corneal epithelium
Front Cell Infect Microbiol. 2023 Oct 5;13:1240903. doi: 10.3389/fcimb.2023.1240903. eCollection 2023. ABSTRACT PURPOSE: In vivo data indicate that mouse corneas exposed to PM10 showed early perforation and thinning after infection with Pseudomonas aeruginosa. To understand the mechanisms underlying this finding, we tested the effects of PM10 and the mitochondria targeted anti-oxidant SKQ1 in immortalized human […]
3D culture induction of adipogenic differentiation in 3T3-L1 preadipocytes exhibits adipocyte-specific molecular expression patterns and metabolic functions
Heliyon. 2023 Oct 5;9(10):e20713. doi: 10.1016/j.heliyon.2023.e20713. eCollection 2023 Oct. ABSTRACT Adipose tissues are closely related to physiological functions and pathological conditions in most organs. Although differentiated 3T3-L1 preadipocytes have been used for in vitro adipose studies, the difference in cellular characteristics of adipogenic differentiation in two-dimensional (2D) culture and three-dimensional (3D) culture remain unclear. In […]
Phenotypic heterogeneity of the mitochondrial DNA A8344G variant presenting with dorsal midbrain syndrome
Am J Ophthalmol Case Rep. 2023 Oct 5;32:101938. doi: 10.1016/j.ajoc.2023.101938. eCollection 2023 Dec. ABSTRACT PURPOSE: To describe a neuro-ophthalmic presentation of a phenotypically heterogeneous mitochondrial DNA variant. OBSERVATIONS: A 10-year-old female with gross motor developmental delay, absence seizures and ataxia subacutely developed poor near acuity and asthenopia. She was found to have accommodative insufficiency, impaired […]
Approaches to the epidemiology of NOHL in the region of Madrid: Survey of neuro-ophthalmologists
Arch Soc Esp Oftalmol (Engl Ed). 2023 Oct 20:S2173-5794(23)00169-X. doi: 10.1016/j.oftale.2023.10.007. Online ahead of print. ABSTRACT OBJECTIVE: To estimate the epidemiology of Leber’s optic neuropathy (NOHL) in the Region of Madrid. MATERIAL AND METHODS: The neuro-ophthalmologists who work at public hospitals of the CAM were interviewed by telephone. They were asked about the number of […]
The murine retinal pigment epithelium requires peroxisomal β-oxidation to maintain lysosomal function and prevent dedifferentiation
Proc Natl Acad Sci U S A. 2023 Oct 24;120(43):e2301733120. doi: 10.1073/pnas.2301733120. Epub 2023 Oct 20. ABSTRACT Retinal pigment epithelium (RPE) cells have to phagocytose shed photoreceptor outer segments (POS) on a daily basis over the lifetime of an organism, but the mechanisms involved in the digestion and recycling of POS lipids are poorly understood. […]
Understanding the complex genetics and molecular mechanisms underlying glaucoma
Mol Aspects Med. 2023 Oct 17;94:101220. doi: 10.1016/j.mam.2023.101220. Online ahead of print. ABSTRACT Glaucoma is the leading cause of irreversible blindness worldwide. Currently the only effective treatment for glaucoma is to reduce the intraocular pressure, which can halt the progression of the disease. Highlighting the importance of identifying individuals at risk of developing glaucoma and […]
Replenishing Age-Related Decline of IRAK-M Expression in Retinal Pigment Epithelium Attenuates Outer Retinal Degeneration
bioRxiv. 2023 Sep 29:2023.09.27.559733. doi: 10.1101/2023.09.27.559733. Preprint. ABSTRACT Unchecked, chronic inflammation is a constitutive component of age-related diseases, including age-related macular degeneration (AMD). Here we identified interleukin-1 receptor-associated kinase (IRAK)-M as a key immunoregulator in retinal pigment epithelium (RPE) that declines with age. Rare genetic variants of IRAK-M increased the likelihood of AMD. IRAK-M expression […]