Real-time monitoring of mitochondrial oxygenation during machine perfusion using resonance Raman spectroscopy predicts organ function
Sci Rep. 2024 Mar 27;14(1):7328. doi: 10.1038/s41598-024-57773-w. ABSTRACT Organ transplantation is a life-saving procedure affecting over 100,000 people on the transplant waitlist. Ischemia reperfusion injury (IRI) is a major challenge in the field as it can cause post-transplantation complications and limit the use of organs from extended criteria donors. Machine perfusion technology has the potential […]
Transketolase promotes MAFLD by limiting inosine-induced mitochondrial activity
Cell Metab. 2024 Mar 23:S1550-4131(24)00082-2. doi: 10.1016/j.cmet.2024.03.003. Online ahead of print. ABSTRACT Metabolic dysfunction-associated fatty liver disease (MAFLD) has a global prevalence of about 25% and no approved therapy. Using metabolomic and proteomic analyses, we identified high expression of hepatic transketolase (TKT), a metabolic enzyme of the pentose phosphate pathway, in human and mouse MAFLD. […]
The Role and Mechanism of Nicotinamide Riboside in Oxidative Damage and a Fibrosis Model of Trabecular Meshwork Cells
Transl Vis Sci Technol. 2024 Mar 1;13(3):24. doi: 10.1167/tvst.13.3.24. ABSTRACT PURPOSE: To investigate the potential effects and mechanism of nicotinamide riboside (NR) on the oxidative stress and fibrosis model of human trabecular meshwork (HTM) cell line cells. METHODS: HTM cells were pretreated with NR, followed by the induction of oxidative injury and fibrosis by hydrogen […]
Diabetic Retinopathy and Regulation of Mitochondrial Glutathione-Glutathione Peroxidase Axis in Hyperhomocysteinemia
Antioxidants (Basel). 2024 Feb 20;13(3):254. doi: 10.3390/antiox13030254. ABSTRACT Diabetic patients have elevated homocysteine levels, and hyperhomocysteinemia is shown to exacerbate mitochondrial damage, which plays a central role in diabetic retinopathy. Glutathione peroxidases (GPx) catalyze hydrogen peroxide (H2O2) reduction using glutathione (GSH) as a cofactor. GSH and GPx are mainly cytosolic but are also present in […]
CASK Mediates Oxidative Stress-Induced Microglial Apoptosis-Inducing Factor-Independent Parthanatos Cell Death via Promoting PARP-1 Hyperactivation and Mitochondrial Dysfunction
Antioxidants (Basel). 2024 Mar 13;13(3):343. doi: 10.3390/antiox13030343. ABSTRACT Calcium/calmodulin-dependent serine protein kinase (CASK) is a scaffold protein and plays critical roles in neuronal synaptic formation and brain development. Previously, CASK was shown to associate with EGFR to maintain the vulval cell differentiation in C. elegans. In this study, we explored the role of CASK in […]
Nucleus-Mitochondria Contact Sites Are Associated With Asynthetic Fission in Zebrafish Skin
Contact (Thousand Oaks). 2024 Mar 14;7:25152564241239445. doi: 10.1177/25152564241239445. eCollection 2024 Jan-Dec. ABSTRACT Rapid increase in body surface area of growing zebrafish larvae (Danio rario) is partially accomplished by asynthetic fission of superficial epithelial cells (SECs) of the skin. There are two cycles of this atypical form of cell division which is unaccompanied by DNA replication; […]
Neuropathic corneal pain: Tear proteomic and neuromediator profiles, imaging features, and clinical manifestations
Am J Ophthalmol. 2024 Mar 21:S0002-9394(24)00117-X. doi: 10.1016/j.ajo.2024.03.015. Online ahead of print. ABSTRACT PURPOSE: To investigate the tear proteomic and neuromediator profiles, in vivo confocal microscopy (IVCM) imaging features, and clinical manifestations in neuropathic corneal pain (NCP) patients. DESIGN: Cross-sectional study. METHODS: Twenty NCP patients and twenty age-matched controls were recruited. All subjects were evaluated […]
CRISPR editing of anti-anemia drug target rescues independent preclinical models of retinitis pigmentosa
Cell Rep Med. 2024 Mar 15:101459. doi: 10.1016/j.xcrm.2024.101459. Online ahead of print. ABSTRACT Retinitis pigmentosa (RP) is one of the most common forms of hereditary neurodegeneration. It is caused by one or more of at least 3,100 mutations in over 80 genes that are primarily expressed in rod photoreceptors. In RP, the primary rod-death phase […]
Sirt3 Protects Retinal Pigment Epithelial Cells From High Glucose-Induced Injury by Promoting Mitophagy Through the AMPK/mTOR/ULK1 Pathway
Transl Vis Sci Technol. 2024 Mar 1;13(3):19. doi: 10.1167/tvst.13.3.19. ABSTRACT PURPOSE: The regulation of mitophagy by Sirt3 has rarely been studied in ocular diseases. In the present study, we determined the effects of Sirt3 on AMPK/mTOR/ULK1 signaling pathway-mediated mitophagy in retinal pigment epithelial (RPE) cells in a high glucose environment. METHODS: The mRNA expression levels […]
Human ocular thelaziasis with genetic analysis in Niigata Prefecture, Japan: A case report on an emerging zoonosis
Am J Ophthalmol Case Rep. 2024 Mar 9;34:102030. doi: 10.1016/j.ajoc.2024.102030. eCollection 2024 Jun. ABSTRACT PURPOSE: We report the clinical findings and molecular identification of ocular Thelazia callipaeda from Niigata Prefecture in the Hokuriku area of Japan during winter. OBSERVATIONS: A 77-year-old male visited an ophthalmology clinic in Niigata Prefecture in January 2022 after a 2-week-duration […]