CRISPR-Cas9-mediated deletion of carbonic anhydrase 2 in the ciliary body to treat glaucoma
Cell Rep Med. 2024 Apr 21:101524. doi: 10.1016/j.xcrm.2024.101524. Online ahead of print. ABSTRACT The carbonic anhydrase 2 (Car2) gene encodes the primary isoenzyme responsible for aqueous humor (AH) production and plays a major role in the regulation of intraocular pressure (IOP). The CRISPR-Cas9 system, based on the ShH10 adenovirus-associated virus, can efficiently disrupt the Car2 […]
Protection against Oxidative Stress by Coenzyme Q10 in a Porcine Retinal Degeneration Model
J Pers Med. 2024 Apr 22;14(4):437. doi: 10.3390/jpm14040437. ABSTRACT Oxidative stress plays an important role in neurodegenerative diseases, including glaucoma. Therefore, we analyzed if the antioxidant coenzyme Q10 (CoQ10), which is also commercially available, can prevent retinal degeneration induced by hydrogen peroxide (H2O2) in a porcine organ culture model. Retinal explants were cultivated for eight […]
Comprehensive Diagnosis, Treatment, and Outcome of Taenia crassiceps Cysticercosis in a Ring-Tailed Lemur (Lemur catta) from a Croatian Zoo: No Longer Unusual?
Pathogens. 2024 Mar 27;13(4):283. doi: 10.3390/pathogens13040283. ABSTRACT Taenia crassiceps is a zoonotic tapeworm of the genus Taenia that is distributed throughout the Northern Hemisphere. Wild and domestic carnivores are final hosts, while rodents and rabbits are primarily intermediate hosts, although many other mammals may harbour the larval stage, Cysticercus longicollis. This case report aims to […]
Effects of Fluorescent Protein tdTomato on Mouse Retina
Exp Eye Res. 2024 Apr 23:109910. doi: 10.1016/j.exer.2024.109910. Online ahead of print. ABSTRACT Fluorescent proteins (FPs) have been widely used to investigate cellular and molecular interactions and trace biological events in many applications. Some of the FPs have been demonstrated to cause undesirable cellular damage by light-induced ROS production in vivo or in vitro. However, […]
THAP3 recruits SMYD3 to OXPHOS genes and epigenetically promotes mitochondrial respiration in hepatocellular carcinoma
FEBS Lett. 2024 Apr 25. doi: 10.1002/1873-3468.14889. Online ahead of print. ABSTRACT Mitochondria harbor the oxidative phosphorylation (OXPHOS) system to sustain cellular respiration. However, the transcriptional regulation of OXPHOS remains largely unexplored. Through the cancer genome atlas (TCGA) transcriptome analysis, transcription factor THAP domain-containing 3 (THAP3) was found to be strongly associated with OXPHOS gene […]
Sirt6 protects retinal ganglion cells and optic nerve from degeneration during aging and glaucoma
Mol Ther. 2024 Apr 23:S1525-0016(24)00253-3. doi: 10.1016/j.ymthe.2024.04.030. Online ahead of print. ABSTRACT Glaucoma is characterized by the progressive degeneration of retinal ganglion cells (RGCs) and their axons, and its risk increases with aging. Yet comprehensive insights into the complex mechanisms are largely unknown. Here, we found that anti-aging molecule Sirt6 was highly expressed in RGCs. […]
TIN2-mediated reduction of mitophagy induces RPE senescence under high glucose
Cell Signal. 2024 Apr 22:111188. doi: 10.1016/j.cellsig.2024.111188. Online ahead of print. ABSTRACT The telomere-associated protein TIN2 localizes to both telomeres and mitochondria. Nevertheless, the impact of TIN2 on retinal pigment epithelial (RPE) cells in diabetic retinopathy (DR) remains unclear. This research aims to examine the role of TIN2 in the senescence of RPE and its […]
Optineurin-facilitated axonal mitochondria delivery promotes neuroprotection and axon regeneration
bioRxiv [Preprint]. 2024 Apr 3:2024.04.02.587832. doi: 10.1101/2024.04.02.587832. ABSTRACT Optineurin (OPTN) mutations are linked to amyotrophic lateral sclerosis (ALS) and normal tension glaucoma (NTG), but a relevant animal model is lacking, and the molecular mechanisms underlying neurodegeneration are unknown. We found that OPTN C-terminus truncation (OPTN∆C) causes late-onset neurodegeneration of retinal ganglion cells (RGCs), optic nerve […]
Targeting dysregulated lipid metabolism for the treatment of Alzheimer’s disease and Parkinson’s disease: Current advancements and future prospects
Neurobiol Dis. 2024 Apr 18:106505. doi: 10.1016/j.nbd.2024.106505. Online ahead of print. ABSTRACT Alzheimer’s and Parkinson’s diseases are two of the most frequent neurological diseases. The clinical features of AD are memory decline and cognitive dysfunction, while PD mainly manifests as motor dysfunction such as limb tremors, muscle rigidity abnormalities, and slow gait. Abnormalities in cholesterol, […]
Molecular mechanism of high glucose-induced mitochondrial DNA damage in retinal ganglion cells
Cell Mol Biol (Noisy-le-grand). 2024 Mar 31;70(3):219-224. doi: 10.14715/cmb/2024.70.3.33. ABSTRACT Mitochondrial DNA damage in retinal ganglion cells (RGCs) may be closely related to lesions of glaucoma. RGCs were cultured with different concentrations of glucose and grouped into 3 groups, namely normal control (NC) group, Low-Glu group, and High-Glu group. Cell viability was measured with cell […]