A photoactivatable chemical lipidomics approach for local sphingolipid metabolic analysis
Methods. 2025 Jul 8:S1046-2023(25)00149-5. doi: 10.1016/j.ymeth.2025.07.002. Online ahead of print. ABSTRACT In eukaryotic cells, lipid metabolism is tightly regulated depending on the subcellular localization, which is essential for maintaining lipid homeostasis. However, understanding compartmentalized lipid metabolism remains challenging due to limited availability of suitable techniques. In this study, we present a chemical lipidomics approach that […]
Leber’s hereditary optic neuropathy-associated ND1 3733G> C mutation ameliorates the mitochondrial quality control and cellular homeostasis
J Biol Chem. 2025 Jul 8:110464. doi: 10.1016/j.jbc.2025.110464. Online ahead of print. ABSTRACT Leber’s hereditary optic neuropathy (LHON) is a paradigm for mitochondrial retinopathy due to mitochondrial DNA (mtDNA) mutations. However, the mechanism underlying LHON-linked mtDNA mutations, especially their impact on mitochondrial and cellular integrity, is not well understood. Recently, the ND1 3733G>C (p.E143Q) mutation […]
Hinokitiol induces developmental and cardiovascular toxicity in zebrafish larvae and potential mechanisms
Toxicol Appl Pharmacol. 2025 Jul 8:117461. doi: 10.1016/j.taap.2025.117461. Online ahead of print. ABSTRACT Hinokitiol is a natural compound collected from the trunk of cypress, belonging to the tropolone family of compounds. It has anti-inflammatory, anti-tumour and antibacterial activities, making it a natural product with a wide range of applications. It is used as an additive […]
Plasma Metabolomics, Lipidomics, and Acylcarnitines Are Associated With Vision and Genotype but Not With Dietary Intake in Long-Chain 3-Hydroxyacyl-CoA Dehydrogenase Deficiency (LCHADD)
J Inherit Metab Dis. 2025 Jul;48(4):e70060. doi: 10.1002/jimd.70060. ABSTRACT 3-hydroxy acylcarnitines (3-OH-ACs) are key biomarkers for screening of long-chain 3-hydroxyacyl-CoA dehydrogenase and trifunctional protein deficiencies (LCHADD/TFPD). The utility of this biomarker for disease monitoring in identified patients remains debated, and recent suggestions have highlighted the potential use of lipidomics for diagnosis, monitoring, prognosis, and/or identification […]
Characterizing Retinal Mitochondrial Function and Its Relations to Intraretinal Thicknesses in Healthy Adults
Curr Eye Res. 2025 Jul 9:1-8. doi: 10.1080/02713683.2025.2527755. Online ahead of print. ABSTRACT PURPOSE: To characterize retinal mitochondrial function and its relationship with intraretinal thicknesses in healthy adults. METHODS: Retinal flavoprotein fluorescence (FPF), a marker of mitochondrial function, was measured using the OcuMet Beacon (OcuSciences, Inc., Ann Arbor, MI), and a stress index (SI) was […]
Longer wavelengths in sunlight pass through the human body and have a systemic impact which improves vision
Sci Rep. 2025 Jul 8;15(1):24435. doi: 10.1038/s41598-025-09785-3. ABSTRACT Long wavelength red light that can extend beyond the human visual range penetrates deeply through biological tissue. Exposure to these longer wavelengths improves mitochondrial function and ATP production. This can translate to improved physiological performance, particularly in the CNS, including the visual system. Light driven metabolic improvements […]
DJ-1 Promotes Diabetic Corneal Epithelial Wound Healing by Attenuating Hyperglycemia-Induced Oxidative Stress Through Inhibiting PTEN
Invest Ophthalmol Vis Sci. 2025 Jul 1;66(9):20. doi: 10.1167/iovs.66.9.20. ABSTRACT PURPOSE: Diabetic keratopathy (DK) is characterized by delayed corneal epithelial wound healing and impaired nerve regeneration, primarily due to mitochondrial oxidative stress. DJ-1 plays a key role in redox regulation. This study explores the effects of DJ-1 downregulation on DK and its mechanisms. METHODS: Type […]
The protective mechanisms of glycyrrhizic acid for deoxynivalenol-induced toxicity in rabbit corneal stroma
Toxicol In Vitro. 2025 Jul 3:106104. doi: 10.1016/j.tiv.2025.106104. Online ahead of print. ABSTRACT OBJECTIVES: This study aimed to investigate the effects and mechanisms of deoxynivalenol (DON) on rabbit corneal stroma cell culture and observe the protective effect of glycyrrhizic acid (GA) against DON-induced damage. METHODS: Rabbit corneal stromal cells (RCSCs) were isolated and divided into […]
Phase 2a/b randomised placebo-controlled dose-escalation trial of triheptanoin for ataxia-telangiectasia: treating mitochondrial dysfunction with anaplerosis
EBioMedicine. 2025 Jul 4;118:105840. doi: 10.1016/j.ebiom.2025.105840. Online ahead of print. ABSTRACT BACKGROUND: Ataxia-telangiectasia (A-T) is a rare multisystem disease characterised by neurodegenerative cerebellar ataxia, lung disease, immune deficiency, high cancer risk, and mitochondrial dysfunction. A-T cells demonstrate defective endoplasmic reticulum-mitochondrial connectivity disrupting calcium homoeostasis and mitochondrial fusion, which are corrected in vitro by the triheptanoin […]
Hypoxia- and mechanical stress-induced upregulation of mitochondrial HSP60 is associated with phenotypic switching of pulmonary arterial smooth muscle cells
Cell Stress Chaperones. 2025 Jul 2:100089. doi: 10.1016/j.cstres.2025.100089. Online ahead of print. ABSTRACT BACKGROUND: Switching from a contractile to a synthetic phenotype of pulmonary arterial smooth muscle cells (PASMCs) is known to play a crucial role in pulmonary arterial hypertension (PAH). We investigated how hypoxia and mechanical stress mediate the phenotypic switching of PASMCs. METHODS: […]