Blockade of interleukin-6 trans-signaling prevents mitochondrial dysfunction and cellular senescence in retinal endothelial cells
Exp Eye Res. 2023 Nov 11:109721. doi: 10.1016/j.exer.2023.109721. Online ahead of print. ABSTRACT Interleukin-6 (IL-6) is a multifaceted cytokine implicated in the pathogenesis of diabetic retinopathy (DR). Its activity extends through cis- and trans-signaling (TS) pathways, with cis-signaling limited to specific cell types possessing the membrane-bound IL-6 receptor, while trans-signaling broadly activates various cells without […]
Potential underlying mechanisms of ethambutol induced optic neuropathy: Evidence from in vitro to clinical studies
Food Chem Toxicol. 2023 Nov 8:114176. doi: 10.1016/j.fct.2023.114176. Online ahead of print. ABSTRACT Ethambutol is an antibiotic widely used for treatment of Mycobacterium species. Although it is safe to use in patients, the ocular toxic impact, including optic neuropathy and retinopathy, can be observed in patients using ethambutol. After discontinuation of the drug, the ocular […]
Glucose Improves the Efficacy of Photobiomodulation in Changing ATP and ROS Levels in Mouse Fibroblast Cell Cultures
Cells. 2023 Oct 27;12(21):2533. doi: 10.3390/cells12212533. ABSTRACT In this study, we tested the idea that photobiomodulation-the application of red to near infrared light (~λ = 600-1300 nm) to body tissues-is more effective in influencing cell metabolism when glucose is readily available. To this end, we used a mouse fibroblast (L-929) cell culture model and had […]
Efficacy of bioactive compounds of Chaihu () on glaucomatous optic atrophy through interleukin-6/hypoxia inducible factor-1α signal pathway
J Tradit Chin Med. 2023 Oct;43(6):1219-1226. doi: 10.19852/j.cnki.jtcm.20230915.001. ABSTRACT OBJECTIVE: To investigate the bioactive compounds of Chaihu (Radix Bupleuri Chinensis) (RB) on glaucomatous optic atrophy (GOA), and to study the pharmacological mechanism. METHODS: We collected information on the bioactive compounds of RB from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP). Targets […]
Spatiotemporal roles of AMPK in PARP-1- and autophagy-dependent retinal pigment epithelial cell death caused by UVA
J Biomed Sci. 2023 Nov 7;30(1):91. doi: 10.1186/s12929-023-00978-4. ABSTRACT BACKGROUND: Although stimulating autophagy caused by UV has been widely demonstrated in skin cells to exert cell protection, it remains unknown the cellular events in UVA-treated retinal pigment epithelial (RPE) cells. METHODS: Human ARPE-19 cells were used to measure cell viability, mitochondrial reactive oxygen species (ROS), […]
De novo variants in RNF213 are associated with a clinical spectrum ranging from Leigh syndrome to early-onset stroke
Genet Med. 2023 Oct 31:101013. doi: 10.1016/j.gim.2023.101013. Online ahead of print. ABSTRACT PURPOSE: RNF213, encoding a giant E3 ubiquitin ligase, has been recognized for its role as a key susceptibility gene for moyamoya disease (MMD). Case reports have also implicated specific variants in RNF213 with an early-onset form of MMD with full penetrance. We aimed […]
Mitophagy and mitochondrion-related expression profiles in response to physiological and pathological hypoxia in the corneal epithelium
Genomics. 2023 Oct 31:110739. doi: 10.1016/j.ygeno.2023.110739. Online ahead of print. ABSTRACT To study the mitochondrial and cellular responses to physiological and pathological hypoxia, corneal epithelial cells were preconditioned under 21% O2, 8% O2 or 1% O2. The cell survival rate, mitochondrial fluorescence and mitophagy flux were quantified using flow cytometry. After RNA sequencing, gene set […]
Single-cell characterization of macrophages in uveal melanoma uncovers transcriptionally heterogeneous subsets conferring poor prognosis and aggressive behavior
Exp Mol Med. 2023 Nov 1. doi: 10.1038/s12276-023-01115-9. Online ahead of print. ABSTRACT Uveal melanoma (UM) is the most frequent primary intraocular malignancy with high metastatic potential and poor prognosis. Macrophages represent one of the most abundant infiltrating immune cells with diverse functions in cancers. However, the cellular heterogeneity and functional diversity of macrophages in […]
Neutral sphingomyelinase inhibition promotes local and network degeneration in vitro and in vivo
Cell Commun Signal. 2023 Oct 30;21(1):305. doi: 10.1186/s12964-023-01291-1. ABSTRACT BACKGROUND: Cell-to-cell communication is vital for tissues to respond, adapt, and thrive in the prevailing milieu. Several mechanisms mediate intercellular signaling, including tunneling nanotubes, gap junctions, and extracellular vesicles (EV). Depending on local and systemic conditions, EVs may contain cargoes that promote survival, neuroprotection, or pathology. […]
Episodic live imaging of cone photoreceptor maturation in GNAT2-EGFP retinal organoids
Dis Model Mech. 2023 Oct 30:dmm.050193. doi: 10.1242/dmm.050193. Online ahead of print. ABSTRACT Fluorescent reporter pluripotent stem cell (PSC) derived retinal organoids are powerful tools to investigate cell type-specific development and disease phenotypes. When combined with live imaging, they enable direct and repeated observation of cell behaviors within a developing retinal tissue. Here, we generated […]