Impaired mitochondrial morphological plasticity and failure of mitophagy associated with the G11778A mutation of LHON

Biochem Biophys Res Commun. 2024 May 14;721:150119. doi: 10.1016/j.bbrc.2024.150119. Online ahead of print.


Mitochondrial dynamics were examined in human dermal fibroblasts biopsied from a confirmed Leber’s Hereditary Optic Neuropathy (LHON) patient with a homoplasmic G11778A mutation of the mitochondrial genome. Expression of the G11778A mutation did not impart any discernible difference in mitochondrial network morphology using widefield fluorescence microscopy. However, at the ultrastructural level, cells expressing this mutation exhibited an impairment of mitochondrial morphological plasticity when forced to utilize oxidative phosphorylation (OXPHOS) by transition to glucose-free, galactose-containing media. LHON fibroblasts also displayed a transient increase in mitophagy upon transition to galactose media. These results provide new insights into the consequences of the G11778A mutation of LHON and the pathological mechanisms underlying this disease.

PMID:38768545 | DOI:10.1016/j.bbrc.2024.150119