Induction of Petite Colonies in Candida glabrate via Rose Bengal-Mediated Photodynamic Therapy

J Vis Exp. 2024 Mar 29;(205). doi: 10.3791/66549. ABSTRACT Facing a 40% mortality rate in candidemia patients, drug-resistant Candida and their petite mutants remain a major treatment challenge. Antimicrobial photodynamic therapy (aPDT) targets multiple fungal structures, unlike antibiotics/antifungals, potentially thwarting resistance. Traditional methods for inducing petite colonies rely on ethidium bromide or fluconazole, which can […]

A challenging differential diagnosis – Leber’s Hereditary Optic Neuropathy

Rom J Ophthalmol. 2024 Jan-Mar;68(1):65-71. doi: 10.22336/rjo.2024.13. ABSTRACT Leber’s hereditary optic neuropathy (LHON) is the most common maternally inherited disease linked to mitochondrial DNA (mtDNA). The patients present with subacute asymmetric bilateral vision loss. Approximately 95% of the LHON cases are caused by m.3460G>A (MTND1), m.11778G>A (MTND4), and m.14484T>C (MTND6) mutations. The hallmark of hereditary […]

Glutaminase deficiency in rod photoreceptors disrupts nonessential amino acid levels to activate the integrated stress response and induce rapid degeneration

bioRxiv [Preprint]. 2024 Mar 27:2024.03.26.582525. doi: 10.1101/2024.03.26.582525. ABSTRACT The bioenergetic demand of photoreceptors rivals that of cancer cells, and numerous metabolic similarities exist between these cells. Glutamine (Gln) anaplerosis via the tricarboxylic acid (TCA) cycle provides biosynthetic intermediates and is a hallmark of cancer metabolism. In this process, Gln is first converted to glutamate via […]

AIBP controls TLR4 inflammarafts and mitochondrial dysfunction in a mouse model of Alzheimer’s disease

bioRxiv [Preprint]. 2024 Mar 27:2024.02.16.580751. doi: 10.1101/2024.02.16.580751. ABSTRACT Microglia-driven neuroinflammation plays an important role in the development of Alzheimer’s disease (AD). Microglia activation is accompanied by the formation and chronic maintenance of TLR4 inflammarafts, defined as enlarged and cholesterol-rich lipid rafts serving as an assembly platform for TLR4 dimers and complexes of other inflammatory receptors. […]

Circumlimbal suture and laser burns: Comparison between two different chronic glaucoma models

Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2024 Jan 28;49(1):84-94. doi: 10.11817/j.issn.1672-7347.2024.230112. ABSTRACT OBJECTIVES: Glaucoma is a multifactorial optic neuropathy with a high rate of irreversible visual loss, and its pathogenesis is complex and still unclear. Elevated intraocular pressure (IOP) is well recognized as the sole modifiable risk factor for the development of […]

A Pilot Analysis of Whole Transcriptome of Human Cryopreserved Sperm

Int J Mol Sci. 2024 Apr 8;25(7):4131. doi: 10.3390/ijms25074131. ABSTRACT Sperm cryopreservation is a procedure widely used to store gametes for later use, to preserve fertility in patients prior to gonadotoxic treatments or surgery, and for sperm donation programs. The purpose of the study was to assess the impact of cryopreservation on human sperm transcriptome. […]

Machine learning-based 3D segmentation of mitochondria in polarized epithelial cells

Mitochondrion. 2024 Apr 8:101882. doi: 10.1016/j.mito.2024.101882. Online ahead of print. ABSTRACT Mitochondria are dynamic organelles that alter their morphological characteristics in response to functional needs. Therefore, mitochondrial morphology is an important indicator of mitochondrial function and cellular health. Reliable segmentation of mitochondrial networks in microscopy images is a crucial initial step for further quantitative evaluation […]

Management of Ptosis in Kearns-Sayre Syndrome: A Case Report and Literature Review

Arch Plast Surg. 2024 Apr 8;51(2):182-186. doi: 10.1055/a-2207-7587. eCollection 2024 Mar. ABSTRACT Kearns-Sayre syndrome (KSS) is a rare mitochondrial disease that affects young adults, due to a deletion of mitochondrial DNA and characterized by the triad: age of onset lower than 20 years, chronic progressive external ophthalmoplegia, and an atypical pigmentary retinopathy. It is also […]

The antioxidant effect of tetrahedral framework nucleic acid-based delivery of small activating RNA targeting DJ-1 on retinal oxidative stress injury

Cell Prolif. 2024 Apr 9:e13635. doi: 10.1111/cpr.13635. Online ahead of print. ABSTRACT Age-related macular degeneration (AMD) and diabetic retinopathy (DR) are the world’s leading causes of blindness. The retinal pigment epithelium (RPE) and vascular endothelial cell exposed to oxidative stress is the major cause of AMD and DR. DJ-1, an important endogenous antioxidant, its overexpression […]