Cytosolic DNA initiates a vicious circle of aging-related endothelial inflammation and mitochondrial dysfunction via STING: the inhibitory effect of Cilostazol
Acta Pharmacol Sin. 2024 Apr 30. doi: 10.1038/s41401-024-01281-0. Online ahead of print. ABSTRACT Endothelial senescence, aging-related inflammation, and mitochondrial dysfunction are prominent features of vascular aging and contribute to the development of aging-associated vascular disease. Accumulating evidence indicates that DNA damage occurs in aging vascular cells, especially in endothelial cells (ECs). However, the mechanism of […]
Deep Learning-Based Identification of Intraocular Pressure-Associated Genes Influencing Trabecular Meshwork Cell Morphology
Ophthalmol Sci. 2024 Mar 5;4(4):100504. doi: 10.1016/j.xops.2024.100504. eCollection 2024 Jul-Aug. ABSTRACT PURPOSE: Genome-wide association studies have recently uncovered many loci associated with variation in intraocular pressure (IOP). Artificial intelligence (AI) can be used to interrogate the effect of specific genetic knockouts on the morphology of trabecular meshwork cells (TMCs) and thus, IOP regulation. DESIGN: Experimental […]
Targeted Metabolomics Highlights Dramatic Antioxidant Depletion, Increased Oxidative/Nitrosative Stress and Altered Purine and Pyrimidine Concentrations in Serum of Primary Myelofibrosis Patients
Antioxidants (Basel). 2024 Apr 19;13(4):490. doi: 10.3390/antiox13040490. ABSTRACT To date, little is known concerning the circulating levels of biochemically relevant metabolites (antioxidants, oxidative/nitrosative stress biomarkers, purines, and pyrimidines) in patients with primary myelofibrosis (PMF), a rare form of myeloproliferative tumor causing a dramatic decrease in erythropoiesis and angiogenesis. In this study, using a targeted metabolomic […]
REGULATORY ROLES OF microRNAs IN MODULATING MITOCHONDRIAL DYNAMICS, AMYLOID BETA FIBRILLATION, MICROGLIAL ACTIVATION, AND CHOLINERGIC SIGNALING: IMPLICATIONS FOR ALZHEIMER’S DISEASE PATHOGENESIS
Neurosci Biobehav Rev. 2024 Apr 24:105685. doi: 10.1016/j.neubiorev.2024.105685. Online ahead of print. ABSTRACT Alzheimer’s Disease (AD) remains a formidable challenge due to its complex pathology, notably involving mitochondrial dysfunction and dysregulated microRNA (miRNA) signaling. This study delves into the underexplored realm of miRNAs’ impact on mitochondrial dynamics and their interplay with amyloid-beta (Aβ) aggregation and […]
CRISPR-Cas9-mediated deletion of carbonic anhydrase 2 in the ciliary body to treat glaucoma
Cell Rep Med. 2024 Apr 21:101524. doi: 10.1016/j.xcrm.2024.101524. Online ahead of print. ABSTRACT The carbonic anhydrase 2 (Car2) gene encodes the primary isoenzyme responsible for aqueous humor (AH) production and plays a major role in the regulation of intraocular pressure (IOP). The CRISPR-Cas9 system, based on the ShH10 adenovirus-associated virus, can efficiently disrupt the Car2 […]
Protection against Oxidative Stress by Coenzyme Q10 in a Porcine Retinal Degeneration Model
J Pers Med. 2024 Apr 22;14(4):437. doi: 10.3390/jpm14040437. ABSTRACT Oxidative stress plays an important role in neurodegenerative diseases, including glaucoma. Therefore, we analyzed if the antioxidant coenzyme Q10 (CoQ10), which is also commercially available, can prevent retinal degeneration induced by hydrogen peroxide (H2O2) in a porcine organ culture model. Retinal explants were cultivated for eight […]
Comprehensive Diagnosis, Treatment, and Outcome of Taenia crassiceps Cysticercosis in a Ring-Tailed Lemur (Lemur catta) from a Croatian Zoo: No Longer Unusual?
Pathogens. 2024 Mar 27;13(4):283. doi: 10.3390/pathogens13040283. ABSTRACT Taenia crassiceps is a zoonotic tapeworm of the genus Taenia that is distributed throughout the Northern Hemisphere. Wild and domestic carnivores are final hosts, while rodents and rabbits are primarily intermediate hosts, although many other mammals may harbour the larval stage, Cysticercus longicollis. This case report aims to […]
Effects of Fluorescent Protein tdTomato on Mouse Retina
Exp Eye Res. 2024 Apr 23:109910. doi: 10.1016/j.exer.2024.109910. Online ahead of print. ABSTRACT Fluorescent proteins (FPs) have been widely used to investigate cellular and molecular interactions and trace biological events in many applications. Some of the FPs have been demonstrated to cause undesirable cellular damage by light-induced ROS production in vivo or in vitro. However, […]
THAP3 recruits SMYD3 to OXPHOS genes and epigenetically promotes mitochondrial respiration in hepatocellular carcinoma
FEBS Lett. 2024 Apr 25. doi: 10.1002/1873-3468.14889. Online ahead of print. ABSTRACT Mitochondria harbor the oxidative phosphorylation (OXPHOS) system to sustain cellular respiration. However, the transcriptional regulation of OXPHOS remains largely unexplored. Through the cancer genome atlas (TCGA) transcriptome analysis, transcription factor THAP domain-containing 3 (THAP3) was found to be strongly associated with OXPHOS gene […]
Sirt6 protects retinal ganglion cells and optic nerve from degeneration during aging and glaucoma
Mol Ther. 2024 Apr 23:S1525-0016(24)00253-3. doi: 10.1016/j.ymthe.2024.04.030. Online ahead of print. ABSTRACT Glaucoma is characterized by the progressive degeneration of retinal ganglion cells (RGCs) and their axons, and its risk increases with aging. Yet comprehensive insights into the complex mechanisms are largely unknown. Here, we found that anti-aging molecule Sirt6 was highly expressed in RGCs. […]